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本院生物化學研究所徐尚德研究員與同所邱繼輝特聘研究員及臺灣大學獸醫專業學院張惠雯副教授共同合作分析臺灣屏東豬下痢冠狀病毒(porcine epidemic diahrrhea virus, PEDV)表面棘蛋白結構功能相關性的研究工作發表於《自然 通訊》Nature Communications https://doi.org/10.1038/s41467-022-32588-3

本研究成果為台灣研究團隊首度利用低溫電子斷層掃描(cryo-electron tomography, cryo-ET)技術取得原子層級之生物巨分子結構資訊。整合低溫電子顯微鏡(cryo-electron microscopy, cryo-EM)分子結構建立技術、質譜分析醣化學組成與蛋白質工程等跨領域研究方法,該團隊明確闡述蛋白質特定位點醣化修飾對於蛋白質功能結構域之構型調控與病毒辨識宿主的相關性。



Porcine epidemic diarrhea virus (PEDV) belongs to the alphacoronavirus family. PEDV is highly contagious and particularly lethal for infected piglets. Despite its apparent threat to the pork industry, Taiwan has no effective vaccination program. The research team led by Dr. Shang-Te Danny Hsu at the Institute of Biological Chemistry, Academia Sinica, in collaboration with Dr. Kay-Hooi Khoo at the same institute, and Prof. Hui-Wen Chang at the School of Veterinary, National Taiwan University, integrated cryo-electron tomography (cryo-ET), cryo-electron microscopy (cryo-EM), mass spectrometry (MS), and protein engineering to reveal the dynamic nature of the spike protein of a highly infectious strain of PEDV identified in Pingtung (PT52). This work has been published online in Nature Communications in August, 2022 (https://doi.org/10.1038/s41467-022-32588-3).

This is the first cryo-ET report from Taiwan that demonstrated the ability to determine atomic structures of biomacromolecules – in this case, the spike proteins as part of intact viral particles. The research team also established a recombinant protein production platform based on a porcine cell line to enable detailed MS analysis of the glycosylation profile of the PEDV spike protein produced by its native host cells. The ability to produce and characterize the molecular details of recombinant PEDV spike proteins will help PEDV vaccine development and disease control in the future.